RESUMO
This article describes the process of building an ontology to assist medical students and professionals specialized in Oncology. The ontology allows the user to obtain knowledge more quickly and thus assist professionals in their decision-making.
Assuntos
Ontologias Biológicas , Neoplasias da Mama/classificação , Mineração de Dados/métodos , Internet/organização & administração , Oncologia/organização & administração , Processamento de Linguagem Natural , Feminino , HumanosRESUMO
Fibropapillomatosis (FP) is a benign neoplasia that affects physiological functions of sea turtles and may lead to death. High prevalence of FP in sea turtle populations has prompted several research groups to study the disease and the associated herpesvirus, chelonid herpesvirus 5 (ChHV5). The present study detected and quantified ChHV5 in 153 fibropapilloma samples collected from green turtles Chelonia mydas on the Brazilian coast between 2009 and 2010 to characterize the relationship between viral load and tumor characteristics. Of the tumor samples collected, 73 and 87% were positive for ChHV5 in conventional PCR and real-time PCR, respectively, and viral loads ranged between 1 and 118.62 copies cell⻹. Thirty-three percent of turtles were mildly, 28% were moderately and 39% were severely affected with FP. Skin samples were used as negative control. High viral loads correlated positively with increasing FP severity in turtles sampled on the Brazilian coast and with samples from turtles found dead in the states of São Paulo and Bahia. Six viral variants were detected in tumor samples, 4 of which were similar to the Atlantic phylogenetic group. Two variants were similar to the western Atlantic/eastern Caribbean phylogenetic group. Co-infection in turtles with more than one variant was observed in the states of São Paulo and Bahia.
Assuntos
Infecções por Herpesviridae/veterinária , Herpesviridae/isolamento & purificação , Papiloma/veterinária , Neoplasias Cutâneas/veterinária , Infecções Tumorais por Vírus/veterinária , Tartarugas , Animais , Brasil/epidemiologia , DNA Viral/análise , Feminino , Herpesviridae/genética , Infecções por Herpesviridae/epidemiologia , Infecções por Herpesviridae/virologia , Masculino , Papiloma/epidemiologia , Papiloma/virologia , Filogenia , Neoplasias Cutâneas/epidemiologia , Neoplasias Cutâneas/virologia , Infecções Tumorais por Vírus/epidemiologia , Infecções Tumorais por Vírus/virologiaRESUMO
The green sea turtles are facing a very high risk of extinction in the wild and the impacts of heavy metals contamination contributes with the decline of their populations. It is very important to assess noninvasive and nonlethal methods for monitoring Hg contamination in sea turtles. Thus, Hg concentrations were measured in keratinized fragments (scutes) and internal tissues of green sea turtles from the Ceará coast to test the usefulness of scutes as a monitoring subject for sea turtles. A significantly positive correlation was found between Hg concentrations in muscle and scutes, which demonstrate that scutes can be used as a predictive matrix of Hg concentration in muscle tissue of green sea turtles.
